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elisa kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology elisa kit
    <t>Serum</t> <t>CXCL12</t> is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via <t>ELISA.</t> The data are presented as the means ± SDs, n=20, **** p < 0.0001
    Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+sdf+1+elisa/Human+SDF-1%2FCXCL12+(Stromal+Cell+Derived+Factor+1)+ELISA+Kit/pmc13021744-42-13-15
    Average 94 stars, based on 24 article reviews
    elisa kit - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway"

    Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

    Journal: Inflammation

    doi: 10.1007/s10753-026-02453-2

    Serum CXCL12 is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via ELISA. The data are presented as the means ± SDs, n=20, **** p < 0.0001
    Figure Legend Snippet: Serum CXCL12 is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via ELISA. The data are presented as the means ± SDs, n=20, **** p < 0.0001

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Anti-Fibrotic Effect of SDF-1β Overexpression in Bleomycin-Injured Rat Lung
    Article Snippet: .. Human SDF-1 ELISA (Cat. No: E-EL-H0052, Elabscience, Wuhan, PRC) was used to measure human SDF-1 levels in rat lung homogenates. .. The rat SDF1/CXCL12 ELISA kit (Cat. No: LS-F23234-1, LS-Bioscience, Seattle, WA, USA) was used to measure SDF-1 levels in rat serum and lung homogenates; to measure TGF-β in rat lung homogenate, rat TGF-β ELISA (Cat. No: EK0514, Boster Bio, Pleasanton, CA, USA) was used.



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    <t>Serum</t> <t>CXCL12</t> is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via <t>ELISA.</t> The data are presented as the means ± SDs, n=20, **** p < 0.0001
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    <t>Serum</t> <t>CXCL12</t> is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via <t>ELISA.</t> The data are presented as the means ± SDs, n=20, **** p < 0.0001
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    <t>Serum</t> <t>CXCL12</t> is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via <t>ELISA.</t> The data are presented as the means ± SDs, n=20, **** p < 0.0001
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    Image Search Results


    CAF‐derived CXCL12 reinforces PDIA6 expression through CXCR4‐STAT3 signaling in GC. (A,B) Immunofluorescence staining and western blot analysis of the CAF markers FAP and α‐SMA in CAFs and paired NFs. (C,D) qRT‐PCR and western blot analyses of PDIA6 mRNA and protein expression in GC cells treated with CAF‐conditioned medium (CAF‐CM) or NF‐conditioned medium (NF‐CM). (E,F) Transwell migration and EdU incorporation assays evaluating the effects of PDIA6 knockdown on CAF‐CM‐induced GC cell phenotypes. (G) Western blot analysis of STAT3 and PDIA6 in GC cells treated with or without CAF‐CM and transfected with control or STAT3 siRNA. (H) RNA‐seq analysis identifying CXCL12 as a highly upregulated secreted factor in CAFs relative to NFs. (I) Correlation analysis of CXCL12 and PDIA6 expression in the TCGA‐STAD cohort. (J,K) Single‐cell RNA‐seq analysis showing fibroblast‐specific expression of CXCL12 in GC tissues. (L,M) Western blot analysis of CXCL12 protein expression and ELISA of CXCL12 secretion in CAFs, NFs, and GC cells. (N) Western blot analysis of p‐STAT3 (Tyr705), total STAT3, and PDIA6 in GC cells pretreated with AMD3100 (2.5 µg/mL) for 30 min and then incubated with or without CAF‐CM for 24 h. (O) Western blot analysis of p‐STAT3 (Tyr705), total STAT3, and PDIA6 in GC cells treated with CAF‐CM together with IgG control or αCXCL12 (10 µg/mL, 24 h). (P) Western blot analysis of p‐STAT3 (Tyr705) and PDIA6 in GC cells treated with CAF‐CM or recombinant human CXCL12 (rhCXCL12, 25/50/100 ng/mL) for 24 h, with or without AMD3100 pretreatment. (Q,R) Cell proliferation and migration assays evaluating the effects of rhCXCL12 stimulation and αCXCL12 neutralization on GC cell behavior. Data are presented as mean ± SD.

    Journal: Advanced Science

    Article Title: PDIA6–SCD1 Axis Rewires Lipid Metabolism to Drive Gastric Cancer Progression

    doi: 10.1002/advs.75923

    Figure Lengend Snippet: CAF‐derived CXCL12 reinforces PDIA6 expression through CXCR4‐STAT3 signaling in GC. (A,B) Immunofluorescence staining and western blot analysis of the CAF markers FAP and α‐SMA in CAFs and paired NFs. (C,D) qRT‐PCR and western blot analyses of PDIA6 mRNA and protein expression in GC cells treated with CAF‐conditioned medium (CAF‐CM) or NF‐conditioned medium (NF‐CM). (E,F) Transwell migration and EdU incorporation assays evaluating the effects of PDIA6 knockdown on CAF‐CM‐induced GC cell phenotypes. (G) Western blot analysis of STAT3 and PDIA6 in GC cells treated with or without CAF‐CM and transfected with control or STAT3 siRNA. (H) RNA‐seq analysis identifying CXCL12 as a highly upregulated secreted factor in CAFs relative to NFs. (I) Correlation analysis of CXCL12 and PDIA6 expression in the TCGA‐STAD cohort. (J,K) Single‐cell RNA‐seq analysis showing fibroblast‐specific expression of CXCL12 in GC tissues. (L,M) Western blot analysis of CXCL12 protein expression and ELISA of CXCL12 secretion in CAFs, NFs, and GC cells. (N) Western blot analysis of p‐STAT3 (Tyr705), total STAT3, and PDIA6 in GC cells pretreated with AMD3100 (2.5 µg/mL) for 30 min and then incubated with or without CAF‐CM for 24 h. (O) Western blot analysis of p‐STAT3 (Tyr705), total STAT3, and PDIA6 in GC cells treated with CAF‐CM together with IgG control or αCXCL12 (10 µg/mL, 24 h). (P) Western blot analysis of p‐STAT3 (Tyr705) and PDIA6 in GC cells treated with CAF‐CM or recombinant human CXCL12 (rhCXCL12, 25/50/100 ng/mL) for 24 h, with or without AMD3100 pretreatment. (Q,R) Cell proliferation and migration assays evaluating the effects of rhCXCL12 stimulation and αCXCL12 neutralization on GC cell behavior. Data are presented as mean ± SD.

    Article Snippet: CXCL12 levels in cell culture supernatants were quantified using a human CXCL12 ELISA kit (RK00266, Abclonal, China) according to the manufacturer's instructions.

    Techniques: Derivative Assay, Expressing, Immunofluorescence, Staining, Western Blot, Quantitative RT-PCR, Migration, Knockdown, Transfection, Control, RNA Sequencing, Single Cell, Enzyme-linked Immunosorbent Assay, Incubation, Recombinant, Neutralization

    Serum CXCL12 is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via ELISA. The data are presented as the means ± SDs, n=20, **** p < 0.0001

    Journal: Inflammation

    Article Title: CXCL12 Promotes Peripheral Nerve Injury Repair by Inhibiting the Ferroptosis-Inflammation Axis via the ERK/Nrf2 Pathway

    doi: 10.1007/s10753-026-02453-2

    Figure Lengend Snippet: Serum CXCL12 is significantly elevated in PNI patients at 72 hours post-operation. The expression level of CXCL12 in the serum was analyzed via ELISA. The data are presented as the means ± SDs, n=20, **** p < 0.0001

    Article Snippet: The samples were then used for the quantitative detection of CXCL12 via an ELISA kit (Elabscience, E-EL-H0052, China).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay